HiSur NHS Beads

HiSur NHS Beads are activated magnetic beads in lyophilized powder form. The NHS activated surface allows covalent amide bond formation to antibodies, proteins, peptides or other biomolecules via primary amine (NH2) groups. Oligonucleotides, antibodies or other ligands with amine groups can be easily coupled to the beads. Attribute to their very large surface area (about three times larger than that of the MonoMag NHS Beads (1 µm) and unique surface coating, HiSur NHS Beads exhibit superior binding capacity and low non-specific binding of protein or nucleic acids.

Ocean NanoTech’s HiSur NHS Beads are also available in an easy-to-use kit format, allowing researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. With the engineered HiSur NHS Beads and the specifically formulated conjugation buffers, the resulting conjugates exhibit high binding capacity and low non-specific binding. Kit contains sufficient reagents & components for performing at least 4 conjugation reactions. Briefly, the NHS beads are mixed in amine-containing molecules such as protein, nuleic acid or small molecules in coupling buffer for 2 hours, then quenching buffer will be added to the solution for 30 min to stop the reaction. After magnetic separation to remove unreacted the molecules, the conjugated beads are stored in storage buffer. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads. Please visit HiSur NHS Beads Conjugation Kit for more details.

Features:

  • Superior binding capacity
  • Significantly low non-specific binding to proteins and nucleic acid
  • Immediate and gentle coupling at room temperature and neutral to low pH
  • Fast magnetic separation
  • Easy to uses

Applications:

  • Immunoprecipitation
  • Nucleic acid isolation
  • Assay development

Product ID Product Name Pricing
Catalog # Product Name Unit Size Unit Price
HN1000-10 HiSur NHS Beads, 1 µm 10 mg $279.00 Add to Cart
  • Weight Percentage: 5%
  • Magnetic Content: ~40%
  • Size: 1 µm (nominal)
Product Name HiSur NHS Beads
Shipping Condition Ice Pack
Storage and Stability -20 °C
             

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