HiSur Carboxyl Beads Conjugation Kit

Ocean NanoTech’s carboxyl terminated HiSur Magnetic Beads (1 µm) are now available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. Attribute to the very large surface area ( about three times larger than that of the MonoMag Carboxyl Beads at the same size (1 µm)) and the unique surface coating of the HiSur Carboxyl Beads as well as the specifically formulated conjugation buffers, the resulting conjugates exhibit superior binding capacity and low non-specific binding. The kit contains sufficient reagents & components for performing at least 5 conjugation reactions. Briefly, the magnetic beads are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.