In order to maximize the binding capacity of our magnetic beads, high density of Maleimide groups are derived based on the covalently bonded BSA. We compared the binding capacity of thiolated IgG for our Maleimide activated magnetic beads and surface activated magnetic beads from other supplier. We found our Maleimide activated magnetic beads have the highest binding capacity for thiolated ligands. The activated magnetic beads could react with thiol groups from targeted protein or ligands in a simple mix-and-go format for use in custom affinity purification experiments. Once the covalent bond is formed, the stable linkage could prevent protein or ligands leaching from the bead surface. The properties of these activated magnetic beads are shown below.
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