In order to maximize the binding capacity of our magnetic beads, high density of NHS groups are derived based on the covalently bonded BSA. We compared all our surface activated beads (NHS activated, Epoxy activated, Tosyl activated, Aldehyde Actuated, divinyl sulfone activated) with the surface activated magnetic beads from other supplier. We found our NHS activated magnetic beads have the highest binding capacity (we discontinue the Epoxy activated, Tosyl activated, Aldehyde Actuated, and divinyl sulfone activated beads now due to their relative low binding capacity). The activated magnetic beads could react with primary amines from targeted protein or ligands in a simple mix-and-go format for use in custom affinity purification experiments. Once the covalent bond is formed, the stable linkage could prevent protein or ligands leaching from the bead surface. The properties of these activated magnetic beads are shown below.
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