Ocean NanoTech’s carboxyl terminated magnetic nanocrystals (MNPs) of various diameters (10 nm-30 nm) are now available in an easy-to-use kit format to enable researchers to conjugate proteins/ligands of their own choice to these MNPs. The kit contains sufficient reagents & components for performing at least 5 conjugation reactions using 1mg MNP per reaction. All componets in the kit have been autoclaved.
Briefly, the MNPs are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The protocol shown below has been used to successfully conjugate bovine serum albumin, streptavidin and immunoglobulin to these MNPs.
|SHP||Iron oxide nanoparticles with carboxylic acid||5-30 nm|
|SYP||Lyophilized (Lyof™) iron oxide nanoparticles with carboxylic acid||5-30 nm|
|SVP||Autoclaved (AclaFTM) iron oxide nanoparticles with carboxylic acid||5-30 nm|
|SHA||Iron oxide nanoparticles with amine||5-30 nm|
|ICK||Carboxyl terminated iron oxide nanoparticles with various diameters (10 nm-30 nm).||10-30 nm|
|SHP-COMBO||A group of 3 or 5 different iron oxide nanoparticles with carboxylic acid||10-30 nm|
|SEI||Iron oxide nanoparticles with positively charged PEI surface||10-30 nm|
|SMG||Iron oxide nanoparticles with PEG ||10-30 nm|
|IRB||Iron oxide nanoparticles with Rhodamine B||10 nm|
|IPG||Iron oxide nanoparticles with Protein G||10-30 nm|
|ILA||Lipid coated iron oxide nanoparticles with amine ||10-30 nm|