Ocean NanoTech’s carboxyl terminated magnetic nanocrystals (MNPs) of various diameters (10 nm-50 nm) are now available in an easy-to-use kit format to enable researchers to conjugate proteins/ligands of their own choice to these MNPs. The kit contains sufficient reagents & components for performing at least 5 conjugation reactions using 1mg MNP per reaction. All componets in the kit have been autoclaved.

Briefly, the MNPs are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The protocol shown below has been used to successfully conjugate bovine serum albumin, streptavidin and immunoglobulin to these MNPs.