SHS is a group of water soluble iron oxide nanoparticles with amphiphilic polymer coating conjugated to streptavidin. Their functional group is streptavidin. The zeta potential of SHS is from -20mV to -40mV. Their organic layers consist of a monolayer of oleic acid and a monolayer of amphiphilic polymer. The thickness of the total organic layers is about 4 nm. The hydrodynamic size of the nanoparticles is about 8-10 nm larger than their inorganic core size measured by TEM. The streptavidin molecules are covalently linked to the polymer. SHS is very stable in most buffer solutions.
SHS can capture biotinylated molecules such as protein, DNA and peptide. For the reactions to link biotinylated molecules onto SHS, it is necessary to use monobiotinylated molecules, which have only one biotin linked to each biomolecule. Otherwise, the nanoparticles will aggregate.
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